Agnieszka Rychlik studied baroque violin with Zygmunt Kaczmarski at the Academy of Music in Cracow and with Lucy van Dael at the Sweelinck Conservatory in Amsterdam. She continued her studies at the Academy of Music in Dresden, with Simon Standage. She also took part in numerous courses of interpretation, led by M. Troost, J. Ter Linden, G. Leonhardt, A. Bylsma, W. Möller, B. Kuijken and others.
Since 1995 she has been a member and deputy leader of the Musicae Antique Collegium Varsoviense, the group associated with the Warsaw Chamber Opera. She also co-operates with many others ensembles of early music, such as Concerto Polacco, the Lower Silesian Baroque Orchestra, Kleine Cammer-Musique, Consortium Iagiellonicum, Harmonia Sacra, Camerata Silesia, Musica Dinamica, Il Tempo, Dancerye, Les Perruques d'Amsterdam and Le Grande Ecurie et la Chambre du Roy.
She has made many recordings in Poland and abroad.